Before Capture Mouse brain with vessels
stained
Captured vessel on LCM cap
Images
courtesy of Dr Helen Ball
University of Sydney Department
of Pathology
Laser Capture Microdissection
Unit
The Laser Capture Microdissection Facility
was opened on 18th August 1999. The advanced microscope is equipped with an
infra red laser, which is used to remove regions of interest from sections
of tissue mounted on microscopic slides. This novel technique allows researchers
to conduct investigations on individual cells using established procedures.
DNA, mRNA and protein can be analysed in laser-captured material.
The
system in use is an Arturus Pix Cell II
Primarily
the unit will function to support and enhance research projects within the University.
In addition, basic research to extend the potential of the microdissecton process
into novel applications will be undertaken. As expertise within the unit develops,
operator training opportunities will become available.
A
number of technical factors contribute to the success, or failure, of microdissection
using this apparatus. As a result, it has been decided that individual access
to the instrument must be restricted initially. Furthermore, as the strategy
for specimen acquisition is driven by the demands of downstream analysis, each
case submitted may need to be discussed in advance, to optimise conditions and
ensure success.
Disposable
accessories required by the process are comparatively expensive, so the unit
is required to operate on a cost
recovery basis.
As interest is expected to be widespread, particularly as the techniques are
revealed by the Literature, potential users are invited to submit expressions
to LCM Laboratory Manager at the below email link. Upon receipt of such advice, arrangements
to discuss the project can commence. The cost of each microdissection is determined
by the extent to which the LCM unit is involved in the project. Consideration
may be given to full project management , subject to the demand on the facility's
resources at the time, and joint ventures to new frontiers are keenly sought.
All enquiries and information should be directed
by email to LCM Manager
or by phone on: 9351 - 6152
