Dr Bob Bao
1. Senior Lecturer, Department of Pathology, University of Sydney, 2001
2. Research Fellow, University of Sydney, 1996-2001
3. Post-doctoral Fellow, University of Sydney 1992-1995
Dr Bao joined the Department of Pathology as a Senior Lecturer in April 2001. His main
research is focusing on mucosal immunity, at both molecular and cellular levels.
1. Cytokine regulation of intestinal mucosal immunity:
The development of two lineages of murine B cells (B1 and B2 cells) in the intestinal mucosa are being investigated, using cytokine gene knock out mice. Although we found that conventional B2 cells are dependent on IL-6, whereas B1 cells rely on IL-5, the precise mechanism(s) underlying these interactions still remains to be explored. More recently, we have been using STAT3 signal transduction gene knock-in mice, and IL-5Rbgene knockout mice, to explore whether these two signal transduction pathways are critical for B2 or B1 cell maturation, respectively.
Collaborator: Professor A Husband, Faculty of Veterinary Science, University of Sydney.
2. Inflammatory bowel disease:
Idiopathic inflammatory bowel disease (IBD) is a significant public health problem in Western societies, affecting 1 in 1000 individuals, yet its aetiology remains poorly understood. It is speculated that mucosal microflora play a role in the initiation and/or perpetuation of the disease process. Using gp91 or iNOS gene knockout mice, the role of these genes in the development of IBD in a mouse model (DSS induced) is being investigated.
Collaborator:
Professor N Hunt, Department of Pathology, University of Sydney.
3. The pathogenesis of atherosclerosis:
Using a microvascular surgical technique, we have successful developed a unique mouse model of endothelial damage/neointimal proliferation that has allowed us to access gene knockout mice to selectively examine the effects of specific proteins in neointimal proliferation. The effects of IFNg, iNOS and other relevant molecules are being investigated.
Collaborator:
Dr B Hambly, Department of Pathology, University of Sydney.
Publications since 1994:
1. Bao S, Goldstone SD and Husband AJ. Localization of IFN_gand IL-6 mRNA in murine intestine by in situ hybridisation. Immunology (1993) 80, 666-671
2. Balcar V, Shen J, Bao S and King, NJC. Na+-dependent high affinity uptake of L-gluta-mate in primary cultures of human fibroblasts isolated from three different types of tissues. FEBS Letter (1994) 339, 50-54
3. Ramsay AJ, Husband, AJ, Ramshaw IA, Bao S, Matthaei K, Kohler G and Kopf M. The role of IL-6 in mucosal IgA responses in vivo. Science (1994) 264, 561-563
4. Husband AJ, Bao S, Muir W, Ramsay AJ and Ramshaw I. Cytokine regulation of mucosal responses: A rational basis for new vaccine delivery strategies. Repro. Fertil. Dev. (1994) 6, 381-388
5. Bao S, dos Remedios CG and King NJC. Ontogeny of MHC antigen expression on cultured human embryonic skeletal myoblasts. Transplantation (1994) 58, 585-591
6. Beagley KW, Bao S, Ramsay AJ, Eldridge JH and Husband AJ. IgA production by peritoneal cavity B cells is IL-6 independent: Implications for intestinal IgA responses. Eur. J. Immunol. (1995) 25, 2123-2126
7. Walker C, Bao S and Canfield PJ. Analysis of dual feline lymphocyte populations observed by flow cytometry. Vet. Immunol., Immunopathol. (1995), 48, 11-25
8. Kramer DR, Sutherland RM, Bao S and Husband AJ. Cytokine mediated effects in mucosal immunity. Immunol. Cell Biol. (1995) 73, 389-396
9. Bao S, Kramer, DR and Husband AJ. The Role of IL-6 in mucosal IgA. Mucosal Immunol. Update (1995) 3, 4-18
10. Bao S, McClure SJ, Emery D and Husband AJ. Detection of IL-5 on eosinophils and g/dT cells in parasite infection in sheep. Eur. J. Immunol. (1996), 26, 552-556
11. Zhou Y, Bao S, Rothwell TL and Husband AJ. IL-5 and eosinophils in parasite infection. Eur. J. Immunol. (1996) 26, 2133-2139
12. Husband AJ, Bao S, McClure SJ, Emery DL and Ramsay AJ. Antigen delivery strategies for mucosal vaccines. Int. J. Parasitol. (1996) 26, 825-834
13. Ramsay AJ, Bao S, Beagley KW, Dustin SJ, Husband AJ, Kopf M, Matthaei K, Ramshaw IA, Shrugnell RA, Young IA and Tan X. Cytokine gene knockout mice - lessons for mucosal B cells development. In: Essentials of mucosal immunology. Kagnoff M. and Kiyono H. Editors (1996)
14. Husband AJ, Kramer DR, Bao S, Sutherland RM and Beagley KW. Regulation of mucosal IgA responses in vivo: Cytokines and adjuvants.Vet. Immunol., Immunopathol. (1997) 54, 179-186
15. Bao S, Beagley, KW, Caristo V, Matthaei K, Young I and Husband AJ. B1 B cells development is IL-5 dependent. Immunology (1998) 94,181-188
16. Cole N, Willcox M, Fleiszig SMJ, Stapleton F, Bao S, Tout S and Husband AJ. Different strains of Pseudomonas aeruginosa isolated from ocular infections or inflammation display distinct corneal pathologies in an animal model. Exp. Eye Research (1998) 18, 730-735
17. Cole N, Willcox MDP, Tout S, Bao S, Stapleton F, Morris CA and Husband AJ. Corneal infection with Pseudomonas aeruginosa causes ulceration and produces IL-6 in infiltrating neutrophils (p327-335). In Mucosal Solutions - Advances In Mucosal Immunology Husband; A Beagley K et al. Editors (1998)
18. Husband A, Bao S and Beagley KW. Differential cytokine regulation of mucosal effector function (p137-144). In Mucosal Solutions - Advances in mucosal immunology Husband A & Beagley K et al. Editors (1998)
19. Lawrence D, Husband AJ Canfield PJ, Allanson, M and Bao S. Elevation of immuno-globulin deposition in the synovial membrane of dogs with cranial cruciate ligament rupture. Vet Immunol Immunopathol (1998) 65, 89-96
20. Bao S, Beagley KW, Allanson M and Husband AJ. Exogenous interleukin-6 promotes enhanced IgA responses in vivo. Immunol. Cell Biol. (1998) 76, 560-562
21. Beagley KW, Bao S and Husband AJ. Mucosal IgA responses in cytokine gene knock-out mice: Differential cytokine requirements for IgA secretion by B1 and B2 cells. Mucosal Immunol Update (1998) 6, 15-19
22. Cole N, Bao S, Willcox, MDP, and Husband AJ. Expression of interleukin-6 in the cornea in response to infection with different strains of Pseudomonas aeruginosa. Infect Immun (1999) 67, 2497-2502
23. Bao S, Husband, AJ and Beagley KW. B1 B cell numbers and antibodies against phosphorylcholine and LPS are increased in IL-6 gene knockout mice. Cell. Immunol, (1999) 198, 139-142
24. Cole N, Bao S, Willcox MDP and Husband AJ. TNF-aproduction in the cornea in response to Pseudomonas aeruginosa challenge. Immunol. Cell Biol. (1999) 77:164-166
25. Shen J, Bao S and Reeve VE. Modulation of IL-10, IL-12 and IFN-gin the epidermis of hairless mice by UVA (320-400 nm) and UVB (280-320 nm) radiation. J. Invest. Dermatol. (1999) 113,1059-1064
26. Beagley KW, Newcombe N, Wu ZL, Gockel CM, Bao S, Holland MJ and Jones RC. Immunoglobulin entry into the male reproductive tract: Implications for immunocon-traception and vaccine development Reproductive Immunology S. K. Gupta editor, Narosa Publishing House New Delhi, India (1999) page 285-293
27. Husband AJ, Bao S and Beagley KW. Analysis of the mucosal microenvironment: factors determining successful responses to mucosal vaccines. Vet Immunol Immunopathol (1999) 72, 135-142
28. Bao S, Beagley KW, France MP, Shen J and Husband AJ. Interferon-gplays a critical role in intestinal immunity against Salmonella typhimurium infection. Immunology, (2000) 99, 464-472
29. Cole N, Bao S, Thakur A, Willcox, M and Husband AJ. KC production in the cornea in response to Pseudomonas aeruginosa challenge. Immunol Cell Biol (2000), 78, 1-4
30. Shen J, Bao S, McClure S, Emery D and Husband AJ. Interleukin-6 expression in gut of parasite challenged sheep. Vet Immunol Immunopathol (2000) 76, 163-168
31. Bao S, Cole N, Willcox M, Beagley KW, Zhou Y and Husband AJ. Differential interleukin-6 mRNA expression in Nippostrongylus brasiliensis infection of susceptible and resistant strains of mice. Immunol Cell Biol (2000) 78, 646-648
32. Shen J, Bao S and Reeve VE. UVA and UVB wavebands modulate expression of fasL in mouse skin epidermis. Redox Report. (2000) 5, 154-155
33. Gockel CM, Bao S and Beagley KW. Transcutaneous immunisation with cholera toxin plus tetanus toxoid induces mucosal and systemic immunity. Mol Immunol (2001) 37; 537-544
34. Wang Y, Wang Y, Feng X, Bao S, Yi S, Kairaitis L, Tay U, Rangan C, Harris CH. Depletion of CD4+ T-cells aggravates glomerular and interstitial injury in murine adriamycin nephropathy. Kidney Int (2001) 59, 975-984
35. Ernst M, Inglese M, Waring P, Clay F, Bao S, Campbell IK, Wicks IP, Alexander WS, Novak U, Tarlinton DM, Heath JK and Dunn AR. Defective gp130 signaling results in synovial chondromatosis, gastrointestinal ulceration and failure of uterine implantation. J Exp Med. (2001) 194, 189-203
36. Cole N, Krockenberger M, Bao S, Beagley KW, Husband A and Willcox M. Effects of exogenous interleukin-6 during pseudomonas aeruginosa corneal infection. Infect Immun, (2001), 69, 4116-4119