This course assumes no prior knowledge of immunohistochemistry and introduces the participants to concepts from the basics.  It would be suitable for both the beginner or those who would like to refresh their knowledge and practical skills in immunohistochemistry.

It is recommended that participants have some prior knowledge of histochemistry or have completed the Introductory Histotechniques course.

The course is completed over two days and consists of a 1 hour lecture in the mornings followed by hands on practical sessions in the laboratory for the remainder of both days.

Concepts covered during the lectures include:

• Fixation for immunohistochemistry
• Direct and indirect immunostaining
• Reporter molecules
• Endogenous enzyme activity
• Blocking techniques
• Double labelling
• Antigen retrieval
• Choice of controls
• Trouble shooting

In the practical sessions participants will:

• Demonstrate smooth muscle actin using the ABC technique
• Restore vimentin immunoreactivity using heat induced antigen retrieval
• Immunofluorescently demonstrate smooth muscle actin using the indirect technique.

Because class size is limited to 10, there is ample opportunity to tailor certain parts to individual needs.  Participants are encouraged to bring along their own antibodies and tissue sections to add to their staining runs or to bring previously attempted slides to trouble shoot with.

Course charges:

• Members of Blackburn/Bosch/QEII Precinct - No charge
• Other members of the University of Sydney - $500.00
• External participants - $650.00

These costs cover all materials used, detailed course notes and lunch or refreshments.

Follow the links on the left of the page to download the Course Registration Form.